Table 2.
Comparison of Method Detection Limit (from OECD TG 456) and Reported LLOQs for HT-H295R
| Hormone Family | Steroid Hormone | OECD TG 456 |
HT-H295R Assay |
|
|---|---|---|---|---|
| Method detection limit (ng/mL) | Lower limit of quantitation (LLOQ) (ng/mL) | Upper limit of quantitation (ULOQ) (ng/mL) | ||
| Androgen | Testosterone (T) | 0.1 | 0.1 | 20 |
| Dehydroepiandrosteronea | NA | 3 | 600 | |
| Androstenedione | NA | 1 | 200 | |
| Estrogen | Estradiol (E2) | 0.01 | 0.03 | 6 |
| Estrone | NA | 0.03 | 6 | |
| Progestagen | 17α-hydroxyprogesterone | NA | 0.2 | 40 |
| 17α-hydroxypregnenolone | 5 | 1000 | ||
| Progesterone | 0.2 | 40 | ||
| Pregnenolonea | 2 | 400 | ||
| Corticosteroid | 11-Deoxycortisol | 5 | 1000 | |
| Deoxycorticosterone | 0.5 | 100 | ||
| Cortisol | 0.5 | 100 | ||
| Corticosterone | 0.5 | 100 | ||
The gray cells highlight the comparison of testosterone (T) and estradiol (E2), as these are the only 2 hormones with minimum method detection limits (MDL) in the performance criteria for the test guidelines. The LLOQs for all of the hormones measured in Karmaus et al. (2016) are listed.
a
Dehydroepiandrosterone and pregnenolone were excluded from further analysis in the work herein as 69.5% and 53.1% of the measured values for these 2 steroid hormones, respectively, were below the LLOQ.