TABLE 3.
Transcriptional assays of vieB
| Growth medium and conditiona | % Tcs CFUb |
|---|---|
| LB broth | 0 |
| M9 salts–0.2% (wt/vol) glucose–0.05% (wt/vol) CAA | 0 |
| Low-iron LB broth | 0 |
| Low-magnesium M9 salts–0.2% (wt/vol) glucose–0.05% CAA | 0 |
| M9 salts lacking NH4+–0.2% (wt/vol) glucose–0.05% CAA | 0 |
| Syncase broth (microaerophilic) | 0 |
| LB broth–0.2 or 0.4% (wt/vol) bile salts | 0 |
| LB broth–1, 10, 20, or 50% (vol/vol) intestinal homogenate | 0 |
| LB broth–5 mM glutathione | 0 |
| Acid shock at pH 8, 7, 6, or 5.7 | 0 |
| AKI (microaerophilic) (ToxR/ToxTON) | 0 |
| CaCo-2 and HCT-8 intestinal cell lines | 0 |
| Mouse intestinal infection | 80 |
a
Growth conditions are described in Materials and Methods. CAA, Casamino Acids.
b
The percent Tcs CFU was determined by replica plating colonies onto LB agar supplemented with 2 μg of tetracycline per ml and is a measure of the transcriptional activity of the vieB::tnpR-lacZY fusion.