Fig. 1.

Acute effects of VPA on insulin secretion and [Ca²⁺]_i in islets and β-cells. a Administration of VPA (200 μg/ml) did not alter insulin secretion from islets under static incubation at 2.8 mM (2.8G) and 8.3 mM glucose (8.3G). Islets were isolated from ICR mice. Values are mean ± SE (n = 9–24 for each group). Gray bar control, black bar VPA. b Elevation of glucose concentration to 8.3 mM increased [Ca²⁺]_i and subsequent administration of VPA (200 μg/ml) failed to alter [Ca²⁺]_i in a single β-cell. c Administration of VPA (200 μg/ml) failed to alter oscillatory [Ca²⁺]_i increase induced by 16.7 mM glucose (16.7G) in a single β-cell. The result is representative of 32 β-cells in (b) and 40 β-cells in (c)