Fig. 2.

Eight-week swimming exercise training increases autophagic flux in mouse skeletal muscle. Representative immunoblot images of LC3, p62, LAMP1 or actin (a). LC3 II/actin (b), LC3 I/actin (c), LC3 II/LC3 I ratio (d), p62/actin (e) and LAMP1/actin (f) ratios were quantitated via densitometry from 8 mice per treatment conditions. Example immunoblot of Beclin-1/Atg6, Atg7 or actin (h). Quantification of Beclin-1/actin (i) and Atg7/actin (j); values are means ± SE; (n = 8) * p < 0.05 vs. sed+sal, ^# p < 0.05 vs. sed+col. Expression of p62 mRNA in triceps muscles of mice was measured following 8-week of swimming exercise training. mRNA levels are relative to the GAPDH (n = 4 per group) (g)