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α-Glycolytic enzymes present in wild-type L. pentosus and LPE1 and LPE2 mutants grown on M medium plus 1% d-xylosea
| Strain | Enzyme activity (nmol/min/mg of protein)
|
|||
|---|---|---|---|---|
| α-p-NPX
|
α-p-NPG
|
|||
| Cytoplasmic fraction | Membrane fraction | Cytoplasmic fraction | Membrane fraction | |
| MD353 | 0 | 52 ± 5 | 464 ± 24 | 44 ± 5 |
| LPE1 | 0 | 0 | 461 ± 14 | 46 ± 5 |
| LPE2 | 0 | 0 | 465 ± 10 | 43 ± 8 |
All experiments were performed in triplicate.
