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. 2016 Sep 9;67(4):497–505. doi: 10.1007/s12576-016-0480-1

Fig. 3.

Fig. 3

TRPC3 regulates migration of C8161 melanoma cells. a Effect of knockdown of TRPC3 on migration distance of C8161 cells, assessed by means of time-lapse video recording (n = 6, **p < 0.01). b Scratch assay showed that Pyr3 significantly inhibited cell migration in a dose-dependent manner (n = 6, **p < 0.01). c Gelatin zymography confirmed that MMP9 secretion was inhibited by ablation of TRPC3 (n = 4, ***p < 0.001). d Pyr3 also dose-dependently decreased MMP9 secretion (n = 4, **p < 0.01). These data suggest that TRPC3 regulates cell migration, i.e., melanoma hematogenous metastasis, via MMP9 secretion