TRPC3 regulates migration of C8161 melanoma cells. a Effect of knockdown of TRPC3 on migration distance of C8161 cells, assessed by means of time-lapse video recording (n = 6, **p < 0.01). b Scratch assay showed that Pyr3 significantly inhibited cell migration in a dose-dependent manner (n = 6, **p < 0.01). c Gelatin zymography confirmed that MMP9 secretion was inhibited by ablation of TRPC3 (n = 4, ***p < 0.001). d Pyr3 also dose-dependently decreased MMP9 secretion (n = 4, **p < 0.01). These data suggest that TRPC3 regulates cell migration, i.e., melanoma hematogenous metastasis, via MMP9 secretion