Fig. 1.
Time-dependent effects of glycosylation inhibition with tunicamycin on CaV3.1-ICa.T. Representative current traces with vehicle and tunicamycin treatment, and group data for current (I)-voltage (V) relationship. HEK-CaV3.1 cells were incubated with vehicle or tunicamycin (10−2 g/l) for 1 h (a), 6 h (b), 12 h (c), or 24 h (d). Changes of the maximum ICa.T (Imax) and the maximum conductance (Gmax) by glycosylation inhibition with tunicamycin are shown as group data (e, f). The ordinate indicates the ratio of the maximum inward current (e) and the maximum conductance (f) with tunicamycin normalized to that with vehicle at the same incubation period. The number of cells is indicated in parentheses. Values represent the mean ± SE. *p < 0.01 vs. the ratio at 1 h. #p = 0.06 vs. the ratio at 12 h. †p = 0.12 vs. the ratio at 12 h