Fig. 4.
Concentration-dependent effects of tunicamycin on half-activation potentials (V1/2-activation) and half-inactivation potentials (V1/2-inactivation). HEK-CaV3.1 cells were incubated for 24 h with vehicle or tunicamycin at the concentration of 10−6 g/l (a), 10−5 g/l (b), 10−4 g/l (c), 10−3 g/l (d), or 10−2 g/l (e). Values for V1/2-activation and V1/2-inactivation were obtained by Boltzmann fittings with the same pulse protocol in Fig. 2. The number of cells is indicated in parentheses. Values represent the mean ± SE. *p < 0.01 vs. vehicle