Fig. 4.

Effects of PG-LPS on myocyte apoptosis and molecules involved in muscle remodeling and autophagy. a Representative images of TUNEL-stained sections of MA in the Control (left) and PG-LPS groups (right). Scale bars: 50 μm. Black arrow: TUNEL-positive myocyte. The number of TUNEL-positive myocytes was significantly increased in the PG-LPS group (far right) (^**P < 0.01 vs Control). b–g Expression of molecules involved in muscle remodeling and autophagy: ERK (Thr-202/Tyr-204) (b), Smad2 (Ser-463/467)/Smad (Ser-423/425) (c), LC3-II (d), Akt (Ser-473) (e), p70S6 K (Thr-389) (f) and 4E-BP1 (Thr-37/46) (g). Expression in the MA of the control group was taken as 100% in each case. Representative immunoblotting results are shown (^*P < 0.05, ^**P < 0.01 vs control). P phosphorylated, T total