Fig. 2.

HDAC4 and HDAC5 levels after 10 days of hindlimb immobilization. Cellular fractionation of proteins (a). Using specific probes, we examined the expression of nuclear Histone H3 or cytosolic SOD1. Representative blots of (b) HDAC4 (c) and HDAC5 (d) in cytosolic (cytosol) and nuclear (nucleus) fractions. Equal protein loading was confirmed by Ponceau S staining, and by GAPDH and Histone H3 immunodetection. Con, n = 11 (white bar); Imm, n = 11 (black bar). All data are expressed as mean ± SE. *p < 0.05 versus Con