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Primers used for the pyrosequencing assay
| Gene | Amplification site | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | *Sequencing primer (5′ to 3′) |
|---|---|---|---|---|
| NFKB2 | −1331 to −1153 | GGGTTTAGGGTAGGGAAAGTTTTT | Biotin-CCCCCCCTAAATAAACCTATCCCCTCT | −1185-TTTAGGAGGGGGGAAT |
The sequencing primer was designed to analyze antisense DNA
