Fig. 6.

Oxidized protein and superoxide dismutase 1 expression. CON, control; LOS, losartan. Oxidized protein (a and b) and superoxide dismutase 1 expression (c and d) on days 1 and 7 after hindlimb unloading. Samples were collected before (PRE), and on days 1 and 7 following hindlimb unloading. Values are represented as mean ± standard deviation; n = 7 per time point. Normally distributed data were analyzed using two-way ANOVA. *P < 0.05 vs. PRE of each sex. For non-normal distribution, the Kruskal–Wallis test was performed, followed by the two-stage step-up method of Benjamini, Krieger, and Yekutieli; the test was used for the analysis within each sex