Fig. 6.

Effect of vidarabine on BO-induced RIP3, phospho-CaMKII and phospho-PLN in the heart of BO mice. a Expression of RIP3, a key mediator of necroptosis, was significantly increased in the BO group (n = 4), but this increase was significantly blocked in the BO + V group (n = 4). ^**P < 0.01, ^***P < 0.001 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Full-size images of immunoblots are presented in Additional file 1: Fig. S13. b Expression of phospho-CaMKII (Thr-286) was significantly increased in the BO group (n = 5), but this increase was significantly blocked in the BO + V group (n = 4). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Full-size images of immunoblots are presented in Additional file 1: Fig. S14. c Expression of phospho-PLN (Thr-17) was significantly increased in the BO group (n = 5), but this increase was blocked in the BO + V group (n = 4). ^*P < 0.05, ^**P < 0.01 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Full-size images of immunoblots are presented in Additional file 1: Fig. S15. d Expression of phospho-PLN (Ser-16) was significantly increased in the BO group (n = 5), but this increase was significantly blocked in the BO + V group (n = 5). ^*P < 0.05, ^**P < 0.01 by one-way ANOVA followed by the Tukey–Kramer post hoc test. Full-size images of immunoblots are presented in Additional file 1: Fig. S16. Data are presented as expressed as mean ± SD