Fig. 1.

Expression of nAChR (α1–7, 9, 10 and β1–4, γ, ε, δ) subunits and mAChR subtypes (m1–m5) in GT1-7 cells. Total RNA from GT1-7 cells was subject to RT-PCR analysis. Expression of nAChR (α1–7, 9, 10 and β1–4, γ, ε, δ) subunit and mAChR subtype (m1–m5) transcripts was examined. Gnrh1 is used as a marker of GnRH neurons. Actb and Gapdh are used as internal controls. Marker 100 bp DNA ladder marker, + reverse-transcribed cDNA, − total RNA without reverse transcriptase, Reference reference organ (the brain section containing the preoptic area and hypothalamus for α2–7, 9, 10, β1–4, γ, ε, and δ, adult skeletal muscle for α1, ε, and δ and neonatal skeletal muscle for γ). The same results were obtained in four separately prepared samples