Fig. 4.

Effects of mAChR agonists on forskolin-induced cAMP formation in GT1-7 cells. GT1-7 cells were incubated in DMEM containing IBMX for 10 min, followed by forskolin and agonist stimulation for 10 min. cAMP concentrations were evaluated using cAMP EIAs. Forskolin-induced cAMP formation was inhibited by 100 nM acetylcholine and 100 nM muscarine. Atropine (100 nM) antagonized the effect of acetylcholine. Dimethyl sulfoxide (0.1 %) was used as a vehicle control. The different letters on the columns indicate statistically significant differences (p < 0.05). The data are represented as the mean ± SEM obtained from four separately prepared samples