Fig. 5.

Effects of nAChRs and mAChRs on GnRH secretion from GT1-7 cells. a Effect of nicotine on GnRH release from GT1-7 cells was assessed using GnRH EIAs. GT1-7 cells were incubated in DMEM with 0.1 % BSA for 15 min and then stimulated by nicotine for 30 min. b Effect of muscarine on IBMX-induced GnRH secretion from GT1-7 cells was assessed using GnRH EIAs. GT1-7 cells were treated with 250 μM IBMX and stimulated by 100 nM muscarine for 6, 12, and 24 h. The different letters on the columns indicate statistically significant differences (p < 0.05). The data were represented as the mean ± SEM obtained from four separately prepared samples