Fig. 3.
a Expression of TRPV5, TRPV6, and Cav1.3 mRNA in Caco-2 monolayer directly exposed for 60 min to 600 ng/mL rhPRL. b GAPDH expression in TRPV5/TRPV6, TRPV5/Cav1.3, TRPV6/Cav1.3 double KD, and TRPV5/TRPV6/Cav1.3 triple KD monolayers. GAPDH expression in scrambled siRNA-treated group was adjusted to 100%. c Representative electrophoretic bands of TRPV5, TRPV6, and Cav1.3 mRNA expression in double and triple KD monolayers. d–f Expression of TRPV5, TRPV6, and Cav1.3 mRNA in double and triple KD Caco-2 monolayers as demonstrated by qRT-PCR. Expression levels were normalized by GAPDH expression. Data are presented as log mean ± SE. *P < 0.05, ***P < 0.001 compared with the scrambled siRNA-treated group. Values of fold differences were also presented along with the statistical symbols. Numbers in parentheses represent the number of independent experiments. Each experiment was performed in triplicate. siTRPV5, siTRPV6, and siCav1.3 mean siRNAs against TRPV5, TRPV6, and Cav1.3 mRNA, respectively