Fig. 4.

Expression of NPR-B in myocardial tissue. a Immunohistochemistry staining of NPR-B in cardiomyocytes. All of the groups of myocardial NPR-B demonstrated a red granular pattern and were located at the cell periphery of the myocardium (original magnification ×400). b Quantitative analysis of NPR-B immunofluorescence. The NPR-B immunofluorescence density values in Group HE, Group EEP, and Group LEP were significantly higher than those in Group C (p < 0.05); compared with Group HE, the NPR-B immunofluorescence density values in Group EEP+HE and Group LEP+HE increased significantly (p < 0.05). c Myocardial NPR-B was measured by western blot analysis. The NPR-B levels in Group HE, Group EEP and Group LEP were significantly higher than those in Group C (p < 0.05); compared with Group HE, the NPR-B levels in Group EEP+HE and Group LEP+HE increased significantly (p < 0.05). The data are presented as the mean ± SD. *p < 0.05 vs Group C, ^# p < 0.05 vs Group HE