Fig. 5.
Dependence of suppression on carbon chain length. The leak current obtained in choline solution was subtracted from all currents. a Current waveform in alcohol solution. Alcohol suppressed voltage-gated Na channel current evoked by 20 ms depolarization to −10 mV from V h of −100 mV. Average from 30 recordings. Upward deflection of bottom trace indicates the timing and duration of the voltage pulse. Black trace control, red trace alcohol, blue trace recovery. Suppression ratio for C4 (1 mM) was −0.31 × 10−1, for C6 (1 mM) was 0.19, and for C8 (0.9 mM) was 0.98. b Suppression ratios for alcohols of different carbon chain length. Suppression ratio for C4 (1 mM) was −0.28 × 10−2 ± 0.37 × 10−1 (n = 3), for C6 (1 mM) was 0.20 ± 0.11 × 10−1 (n = 4), and for C8 (0.9 mM) was 1.07 ± 0.81 × 10−1 (n = 3). Using t test for **1 and **2, p < 0.005. c Relationship between suppression ratio and carbon chain length. Left: black squares show C8 alcohol (0.07 mM), C6 alcohol (0.1 mM), and C4 alcohol (0.1 mM). Red circles show C8 ester (0.08 mM), C6 ester (0.1 mM), and C4 ester (0.1 mM). Blue triangles show C6 carboxylic acid (0.1 mM). Center: black squares show C8 alcohol (0.9 mM), C6 alcohol (1 mM), and C4 alcohol (1 mM). Red circles show C8 ester (0.8 mM), C6 ester (1 mM), and C4 ester (1 mM). Blue triangles show C8 carboxylic acid (0.8 mM), C6 carboxylic acid (1 mM), and C4 carboxylic acid (1 mM). Right: black squares show C6 alcohol (10 mM) and C4 alcohol (10 mM). Red circles show C8 ester (2.1 mM), C6 ester (10 mM), and C4 ester (10 mM). Blue triangles show C8 carboxylic acid (2.5 mM), C6 carboxylic acid (10 mM), and C4 carboxylic acid (10 mM). Error bars show SD. Numbers in parentheses indicate the number of trial cells