Fig. 3.

Effects of neonatal estrogenization and prepubertal gonadectomy on Kiss1 mRNA expression in anteroventral periventricular nucleus and periventricular nucleus continuum (AVPV/PeN) of prepubertal rats. a Representative photographs of Kiss1 mRNA expressing neurons in AVPV/PeN of PND18 males that were GDX or left intact on PND14 and females that received oil or EB injection on PND0 and were GDX or left intact on PND14. 3V Third ventricle. Scale bar 200 μm. b Mean number of Kiss1 mRNA expressing neurons in AVPV/PeN of each experimental group. Error bars SEM. Bars labeled with different letters are significantly different from each other (p < 0.05). Asterisks indicate significant differences between intact and GDX groups (p < 0.05)