Fig. 5.

MiR-200c-3p promoted EndMT in ox-LDL-treated HUVECs by inhibiting YAP expression. HUVECs were co-transfected with miR-200c-3p mimic or mimic NC and pcDNA3.1-YAP or pcDNA3.1-NC, followed by 40 μg/mL ox-LDL treatment. A The expression of YAP in the HUVECs was assessed by WB. B Rhodamine phalloidin staining was performed to observe cell morphology of HUVECs. The blue arrows indicate cobblestone-like cells, and the red arrows indicate the spindle-like cells. C WB was performed to estimate the expression of CD31, vWF, α-SMA, and vimentin in the HUVECs. **P < 0.01 vs. mimic NC + Vector group; ^##P < 0.01 vs. mimic NC + YAP group