Fig. 6.

RT-PCR analysis of MT1 and MT2 mRNA expression in various brain subregions and peripheral organs. Total RNA isolated from various brain subregions and peripheral organs was subjected to RT-PCR using specific primers for MT1, MT2 and β-actin cDNAs. a Expression of MT1 (upper panel), MT2 (middle panel), and β-actin (lower panel) in rat brain subregions. b Expression of MT1 (upper panel), MT2 (middle panel) and β-actin (lower panel) in rat whole brain and peripheral organs. The number on the right of each panel indicates the number of PCR cycles performed. Similar patterns of expression were observed in three different samples