Table 1.
General electrical properties of the two groups of AFSC approached via whole-cell patch-clamp
| Feature | R p (MΩ) | C m (pF) | R m (MΩ) | R a (MΩ) | τ (μs) | I hold (pA) | Preservation of the seal at the end of exp. |
|---|---|---|---|---|---|---|---|
| Senescent AFSC (n = 18) | |||||||
| Mean | 2.03 | 47.27 | 2021.34 | 7.62 | 370.55 | −179.17 | 72.22 % |
| SD | 0.83 | 22.28 | 2253.96 | 4.44 | 291.41 | 295.54 | |
| Cryopreserved AFSC (n = 19) | |||||||
| Mean | 2.33 | 44.97 | 1519.67 | 8.92 | 415.46 | −100.95 | 78.94 % |
| SD | 0.77 | 26.75 | 1927.00 | 4.24 | 330.09 | 95.71 | |
R p pipette resistance, C m membrane capacitance, R m membrane resistance, R a access resistance, τ time constant of the equivalent electrical circuit, I hold holding current at −80 mV