Fig. 4.

Effect of hypoxia on myocardial catalase and aldehyde dehydrogenase-2. Expression levels of mRNA and protein of CAT and ALDH-2 in LV preparations from SHR and SHR-mtBN rats kept in normoxic (empty bars) and hypoxic (hatched bars) conditions were assessed by RT-PCR and Western blotting, respectively. The amounts of specific mRNA species were normalized with 18S levels (column a). The 18S levels were not significantly affected by hypoxic exposure. Representative Western blots are shown below the corresponding bar graphs depicting the relative protein expression levels (column b). Data represent means  (±SEM) of at least three separate experiments. ⁺ p < 0.05 SHR vs. SHR/H rats; ^§ p < 0.05 SHR/H vs. SHR-mtBN/H rats; ^# p < 0.05 SHR-mtBN vs. SHR-mtBN/H rats