Table 1 .
Sequence of primers for real time PCR and Universal Probe Library (UPL) probes
| Gene | Forward (F) and reverse (R) primer | UPL probes |
|---|---|---|
| SOD-1 |
F:5′-GGTCCAGCGGATGAAGAG-3′ R:5′-GGACACATTGGCCACACC-3′ |
5 |
| GPx-4 |
F:5′-TGGGAAATGCCATCAAATG-3′ R: 5′-CGGCAGGTCCTTCTCTATCA-3′ |
25 |
| Catalase |
F:5′-ATCAGGGATGCCATGTTGTT-3′ R: 5′-GGGTCCTTCAGGTGAGTTG-3′ |
129 |
| Cytochrome c oxidase |
F:5′-AGCCAAATCTCCCACTTCC-3′ R: 5′-ATAGCTCTCCAAGTGGGATAAGAC-3′ |
18 |
| Cav1.2, α1c-subunit |
F:5′-GAGGGCTGGACAGACGTG-3′ R:5′-TGACCCTATGATGATTAGTGTTACAAA-3′ |
81 |
| SERCA-2 |
F:5′-ATGGACGAGACGCTCAAGTT-3′ R:5′-GTTTAGGAAGCGGTTACTCCAG-3′ |
1 |
| Calsequestrin |
F:5′-ATTTGCGGAGAAGAGTGACC-3′ R: 5′-CCGGGCAACCTGTTTTAG-3′ |
80 |
| Na+-K+-ATPase, α1-subunit |
F:5′-CACCAAGATAGTGGAGATTCCTTT-3′ R:5′-TGGGTTCTTGTGAATGGAGA-3′ |
22 |
| Collagen-1 |
F:5′-CTGGCAACCTCAAGAAGTCC-3′ R: 5′-CAAGTTCCGGTGTGACTCG-3′ |
65 |
| TGF-β1 |
F:5′-TCAGACATTCGGGAAGCAGT-3′ R: 5′-ACGCCAGGAATTGTTGCTAT-3′ |
56 |
| β-actin |
F:5′-CTAAGGCCAACCGTGAAAAG-3′ R: 5′-TACATGGCTGGGGTGTTGA-3′ |
115 |
| GAPDH |
F:5′-CTACCCACGGCAAGTTCAAT-3′ R: 5′-ATTTGATGTTAGCGGGATCG-3′ |
111 |
SOD-1 Superoxide dismutase-1, GPx-4 glutathione peroxidase-4, α1c-subunit; Ca v 1.2 L-type calcium-channel, SERCA-2 sarco(endo)plasmic 2 Ca2+-ATPase-2, Na + -K + -ATPase sodium–potassium adenosine triphosphatase, TGF-β 1 transforming growth factor β1, GAPDH glyceraldehyde 3-phosphate dehydrogenase