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Acetylcholine (ACh)-induced activity of Rho-associated protein kinase (ROCK) in GSMC isolated from male and female rats. ROCK activity is expressed as absorbance at 450 nm. Treatment of GSMC with ACh (0.1 µM) significantly increased ROCK activity in the cells of both sexes. Note that the ACh-induced activation of ROCK was significantly higher in cells isolated from male rats than in those isolated from female rats (*P < 0.01 vs. female; n = 10 males, n = 7 females)
