Fig. 4.

Circadian expression profiles of Per2, Bmal1, Dbp, and Aqp5 mRNA in cultured SMGs ex vivo. a–c Per2 (a), Bmal1 (b), and Dbp (c) mRNA expression levels in SMG cultures prepared at ZT 0 (gray column) and ZT 12 (black column). Samples at ZT 8, 12, and 16 were collected from a ZT 0 preparation, and samples from ZT 20, 24, and 4 were collected from a ZT 12 preparation. d–f Aqp5 mRNA expression levels in samples (d) prepared at ZT 0 (e) and ZT 12 (f). Samples were collected at the projected ZTs described on each chart. Data are shown as mean ± SEM (n = 5 mice per time point in ZT 0 preparation and n = 6 mice per time point in ZT 12 preparation)