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. 1998 May;180(9):2359–2366. doi: 10.1128/jb.180.9.2359-2366.1998

FIG. 1.

FIG. 1

Transcription template plasmid with rRNA P1 test promoter and SP6 control promoter. Wild-type rRNA P1 (−302 to +5), or promoter templates containing mutations, was cloned immediately upstream of the test G-less cassette in plasmid pMT589. In vitro transcription with C. trachomatis RNAP or E. coli RNAP produced a 158-nucleotide (nt) test transcript. The control promoter region contained an SP6 promoter cloned in front of the control G-less cassette. Transcription with SP6 RNAP produced a 130-nucleotide control transcript.