Effects of single substitutions on C. trachomatis rRNA P1 transcription by E. coli or C. trachomatis RNAPs. All three possible substitutions were tested from −41 to −1, except −41, −19, −18 and −16, where only two were tested (Table 1). The wild-type rRNA P1 sequence is shown on each graph, and changes in promoter activity resulting from the substitutions, relative to the activity of the wild-type promoter, are indicated. Decreases larger than fivefold are not illustrated as extending beyond the bottom axis.