FIG. 6.

In vitro transcription using wild-type E. coli RNAP (α) or E. coli RNAP with a deletion of the α-CTD (α-del 235). C. trachomatis rRNA P1 templates contained an UP element (wild type [wt]; −302 to +5) or lacked an UP element (Δ34; −34 to +5). For the Δ34 template, the sequence that replaced the UP element was derived from the plasmid (sequence in Δ34 from −64 to −35: GTCGCATGCTCCTCTAGACTCGAGGAATTC. The E. coli lacUV5 promoter, which lacks an UP element (17), was used to normalize the activities of the two RNAP preparations.