TABLE 1.

Promoter activities of rRNA P1 templates with single base substitutions

Relative promoter activity ± SDa
Position	Wild-type sequence	C. trachomatis RNAP				E. coli RNAP
		A	C	G	T	A	C	G	T
−1	T	105 ± 9	64 ± 7	107 ± 17	100	118 ± 3	108 ± 5	96 ± 3	100
−2	T	69 ± 3	66 ± 3	77 ± 18	100	79 ± 6	116 ± 2	120 ± 2	100
−3	G	118 ± 5	92 ± 5	100	133 ± 14	131 ± 8	63 ± 1	100	80 ± 2
−4	T	47 ± 3	53 ± 9	80 ± 19	100	15 ± 4	20 ± 5	34 ± 13	100
−5	A	100	92 ± 27	54 ± 4	65 ± 4	100	86 ± 8	42 ± 1	32 ± 3
−6	G	152 ± 30	126 ± 8	100	177 ± 23	123 ± 5	109 ± 0	100	171 ± 14
−7	A	100	43 ± 7	48 ± 19	113 ± 15	100	37 ± 3	37 ± 2	94 ± 7
−8	A	100	28 ± 4	11 ± 1	48 ± 5	100	6 ± 3	6 ± 4	9 ± 2
−9	T	21 ± 5	72 ± 8	49 ± 5	100	8 ± 2	12 ± 2	10 ± 4	100
−10	A	100	58 ± 7	160 ± 10	138 ± 4	100	46 ± 3	108 ± 8	52 ± 1
−11	T	102 ± 6	65 ± 8	103 ± 7	100	25 ± 2	47 ± 8	70 ± 4	100
−12	A	100	116 ± 11	112 ± 4	117 ± 23	100	68 ± 3	121 ± 3	94 ± 5
−13	G	106 ± 17	95 ± 24	100	144 ± 25	76 ± 1	67 ± 8	100	30 ± 3
−14	T	90 ± 4	68 ± 8	77 ± 8	100	55 ± 2	32 ± 2	55 ± 3	100
−15	T	146 ± 20	141 ± 15	129 ± 9	100	103 ± 29	90 ± 5	109 ± 5	100
−16	G	144 ± 13	96 ± 15	100	Not tested	124 ± 13	102 ± 6	100	Not tested
−17	G	115 ± 10	118 ± 9	100	106 ± 14	161 ± 6	100 ± 7	100	149 ± 3
−18	A	100	117 ± 26	117 ± 13	Not tested	100	116 ± 5	112 ± 9	Not tested
−19	G	137 ± 18	Not tested	100	93 ± 21	146 ± 14	Not tested	100	123 ± 9
−20	A	100	101 ± 15	109 ± 9	90 ± 5	100	118 ± 16	108 ± 7	105 ± 8
−21	T	97 ± 9	106 ± 14	103 ± 17	100	87 ± 6	104 ± 7	106 ± 8	100
−22	A	100	52 ± 6	85 ± 11	83 ± 18	100	78 ± 11	121 ± 3	72 ± 0
−23	A	100	27 ± 3	31 ± 3	130 ± 8	100	69 ± 6	73 ± 5	121 ± 5
−24	A	100	22 ± 3	32 ± 6	70 ± 8	100	115 ± 11	155 ± 4	99 ± 1
−25	A	100	51 ± 9	53 ± 10	126 ± 18	100	141 ± 13	81 ± 6	144 ± 6
−26	A	100	65 ± 4	94 ± 7	141 ± 13	100	98 ± 8	113 ± 16	140 ± 10
−27	A	100	101 ± 14	72 ± 6	79 ± 7	100	82 ± 2	49 ± 10	66 ± 8
−28	G	153 ± 19	100 ± 4	100	120 ± 19	176 ± 5	149 ± 10	100	127 ± 6
−29	A	100	108 ± 5	147 ± 19	103 ± 5	100	69 ± 5	67 ± 5	43 ± 6
−30	C	119 ± 7	100	87 ± 6	137 ± 46	78 ± 3	100	167 ± 9	118 ± 7
−31	G	92 ± 9	68 ± 5	100	116 ± 15	109 ± 14	48 ± 5	100	115 ± 2
−32	T	85 ± 15	49 ± 3	52 ± 10	100	48 ± 20	32 ± 4	36 ± 7	100
−33	A	100	126 ± 14	110 ± 12	148 ± 23	100	85 ± 2	79 ± 3	242 ± 18
−34	G	79 ± 7	96 ± 20	100	106 ± 8	58 ± 5	58 ± 5	100	75 ± 9
−35	A	100	130 ± 26	121 ± 20	85 ± 17	100	56 ± 6	68 ± 7	67 ± 5
−36	T	117 ± 15	116 ± 12	123 ± 12	100	111 ± 4	86 ± 2	89 ± 1	100
−37	A	100	103 ± 19	105 ± 16	175 ± 8	100	70 ± 6	105 ± 3	70 ± 6
−38	A	100	66 ± 7	88 ± 18	109 ± 16	100	46 ± 7	50 ± 5	65 ± 5
−39	A	100	89 ± 16	95 ± 15	126 ± 26	100	50 ± 8	53 ± 7	88 ± 6
−40	A	100	99 ± 20	125 ± 27	109 ± 18	100	76 ± 4	104 ± 8	95 ± 4
−41	A	100	Not tested	116 ± 6	79 ± 4	100	Not tested	83 ± 8	65 ± 4

^aThe promoter activity of each mutant promoter was normalized to a wild-type rRNA P1 activity of 100%. Each value is the mean of three separate measurements.