Fig. 1.

Swelling-activated whole-cell currents in mouse colonic epithelial MCE301 cells. a Representative whole-cell currents induced by hypotonic stimulation. Alternating pulses of ±40 mV and step pulses from −100 to +100 mV in 20-mV increments (with a pre-pulse to −100 mV and a post-pulse to −60 mV) were applied from a holding potential of 0 mV. Daggers Time points at which step pulses were applied, arrowhead the zero current level. b Expanded traces of current responses to step pulses (inset) applied in a under isotonic (left), hypotonic (middle), and re-isotonic (right) conditions. c Instantaneous current–voltage (I–V) relationships of swelling-activated currents under isotonic, hypotonic, and re-isotonic conditions. I (pA/pF): current density, Vm: membrane potential. Each data point represents the mean ± standard error of the mean (SEM; vertical bar) of five experiments. *P < 0.05 compared to the currents under isotonic conditions