Fig. 4.

Effects of Cl⁻ channel blockers on apoptotic processes of MCE301 cells treated with sodium butyrate for 2 days. a Representative dot-plots showing the distribution of cells stained with annexin V and/or propidium iodide (PI). Annexin V- and PI-positive cells (upper right) are defined as late apoptotic cells. b, c Quantification of late apoptotic cells: 2.5 µM DCPIB (b) and 10 µM NPPB (c) decreased the number of late apoptotic cells after exposure to 8 mM sodium butyrate for 2 days. Each column represents the mean ± SEM (vertical bar) of five experiments. *P < 0.05 compared to control, ^# P < 0.05 compared to butyrate. FITC Fluorescein isothiocyanate