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Immunostaining of LC3 and Atg12, and visualization of lysosomes. a, b Confocal laser scanning microscopy of double-immunostaining of LC3 (green) and α-actinin (red), DAPI staining (blue) and DIC images of ACMs cultured for 3 (a) and 6 (b) days. c, d Immunostaining of Atg12 of ACMs cultured for 3 (c) and 6 (d) days. e Fluorescent staining of lysosomes with LysoTracker (red) and nuclei with Hoechst 33342 (blue) in beating ACMs cultured for 6 days. Bars (a, c) 25 µm, (b, d, e) 50 µm
