Table 1.

PCR primers, conditions and sizes of PCR products

Gene name	Protein name	Accession no.	Primer	Temp (°C)a	Cycleb	Size (bp)c
			Sense Antisense
Ctsb	CathepsinB	BC072490	5’-GAAGCCATGTCTGACCGAAT-3’ 5’-CCACTGGGCCATTTTTGTAG-3’	60	28	406
Fstl1	Follistatin-like 1	NM_024369	5’-GCCATGGAGACCTAACCAAA-3’ 5’-TGAGGGACCTCTTGTGCTTT-3’	56	32	386
Fyn	Src kinase p59	NM_012755	5’-GCTTGGATAATGGGCTGTGT-3’ 5’-CATCTTCTGTCCGTGCTTCA-3’	60	34	306
Grin2b	Glutamate ionotropic receptor	NM_012574	5’-CCCAAGTTCTGGTTGGTGTT-3’ 5’-GATCTGAGCGATGGCTTCTT-3’	62	40	303
Itpr1	Inositol1,4,5-triphosphate	NM_001007235	5’-GGTTGTGAACTTAGCCAGGAAC-3’ 5’-CTGGTACATTACTTGGCCCTTC-3’	58	36	481
Map2k1	Mitogen-activated protein kinase	NM_031643	5’-GCAAGATGCCCAAGAAGAAG-3’ 5’-GATGATCTGGTTCCGGATTG-3’	60	34	339
Npy	Neuropeptide Y	NM_012614	5’-GCTAGGTAACAAACGAATGGGG-3’ 5’-CACATGGAAGGGTCTTCAAGC-3’	55	37	288
S100a10	Protein S100-A10 (p11)	BC091565	5’-TGCTCATGGAAAGGGAGTTC-3’ 5’-GCTTGTCGAATTGGAGTTGG-3’	60	30	330
St8sia1	GD3 synthase	NM_012813	5’-GGAAAGCCAAAGGTGTGTGT-3’ 5’-GGTTGCAGCAGTCTTCCATT-3’	56	34	373
Tac 1	Substance P	NM_012666	5’-CGAGCAGTGAGCGGTAAAAT-3’ 5’-CGCAAGACACACAGGAGTTT-3’	60	27	305
CypA	Peptidylprolyl isomerase A (cyclophilin A) (internal standard)	BC059141	5’- ACGCCGCTGTCTCTTTTC -3’ 5’- TGCCTTCTTTCACCTTCC -3’	55	24	440

^aTemp: annealing temperature

^bCycle: PCR cycles

^cSize: sizes of PCR products