Table 2.
RT-PCR of the pain-related genes whose expression changes were detected by PCR-based cDNA subtraction or DNA microarray analysis
| Gene name | Protein name | Assay | Direction | RT-PCR | |
|---|---|---|---|---|---|
| (Fold of increase or decrease) | Direction | Fold of changes | |||
| Ctsb | CathepsinB | Subtraction | Down | Down | 0.9 |
| Fstl1 | Follistatin-like 1 | Subtraction | Down | Down | 0.8 |
| Fyn | Src kinase p59 | Microarray | Up (2.7) | Up | 1.7 |
| Grin2b | Glutamate ionotropic receptor | Microarray | Up (2.6) | non | – |
| Itpr1 | Inositol 1,4,5-triphosphate receptor, type 1 | Microarray | Down (0.5) | Down | 0.6 |
| Map2k1 | Mitogen-activated protein kinase | Microarray | Up (2.6) | non | – |
| Npy | Neuropeptide Y | Microarray | Down (0.5) | Down | 0.7 |
| S100a10 | Annexin 2 light | Subtraction | Down | Down | 0.5 |
| St8sia1 | GD3 synthase | Microarray | Up (2.4) | Up | 1.3 |
| Tac 1 | Substance P | Subtraction | Up | Up | 3 |
Microarray, DNA microarray analysis; subtraction, PCR-based cDNA subtraction