Fig. 3.

Piezo1 protein expression in mouse interscapular brown adipose tissue. A RT-PCR analysis of Piezo1 and Act-b expression in mouse interscapular brown adipose tissue (iBAT) from 6-week-old male wild-type (WT) mice. RT ( +) and (−) indicate the samples treated with and without reverse transcriptase, respectively. B Morphological image of iBAT from 6-week-old male WT mice stained with hematoxylin and eosin (H&E). C Immunohistochemical image of iBAT from 6-week-old male WT mice in the absence of the primary antibody (Control). D and E Immunohistochemical images of iBAT from 6-week-old male WT mice using an anti-Piezo1 (D) or anti-UCP1 (E) antibody. Scale bar: 50 μm