Table 1.

Sequences of the primers for RT-PCR and RT-qPCR

Gene	Forward primer (5′–3′)	Reverse primer (5′–3′)
Piezo1 (RT-PCR)	CGGAACCTGACCTTGACAAC	CCAACTGGTGCAGGCTGAC
Act-b (RT-PCR)	ACCCGCGAGCACAGCTTCT	ATCACACCCTGGTGCCTA
Ucp1 (RT-qPCR)	CAAAGTCCGCCTTCAGATCC	AGCCGGCTGAGATCTTGTTT
Piezo1 (RT-qPCR)	ATCCTGCTGTATGGGCTGAC	AAGGGTAGCGTGTGTGTTCC
Pparγ (RT-qPCR)	GTGCCAGTTTCGATCCGTAGA	GGCCAGCATCGTGTAGATGA
Prdm16 (RT-qPCR)	CAGCACGGTGAAGCCATTC	GCGTGCATCCGCTTGTG
Pgc1α (RT-qPCR)	CCCTGCCATTGTTAAGACC	TGCTGCTGTTCCTGTTTTC
Caspase-3 (RT-qPCR)	GGAGCTTGGAACGGTACGC	CACATCCGTACCAGAGCGAG
Bax (RT-qPCR)	GAGCTGCAGAGGATGATTGC	CTTGGATCCAGACAAGCAGC
Bcl-2 (RT-qPCR)	GTCGCTACCGTCGTGACTTC	CTGGGGCCATATAGTTCCACAA
36b4 (RT-qPCR)	GGCCCTGCACTCTCGCTTTC	TGCCAGGACGCGCTTGT