Fig. 1.

Immunostaining for insulin and nesfatin-1 in pancreatic islets and hypothalamic arcuate nucleus of Cre and βNUCB2 KO mice. a Double immunostaining for insulin and nesfatin-1 in islets. The upper rows show Cre mice and the lower rows βNUCB2 KO mice. Left: insulin (green: Alexa 488 fluorescence), middle: nesfatin-1 (red: Alexa 594), right: merged images. n = 21 islet sections from six Cre mice, and n = 24 islet sections from seven βNUCB2 KO mice. b Immunostaining for nesfatin-1 (green: Alexa 488) in hypothalamic arcuate nucleus. Upper and lower panels show Cre and βNUCB2 KO mice, respectively. n = 9 sections from one Cre mice, and n = 9 sections from one βNUCB2 KO mice. Scale bars: 50 μm (color figure online)