Fig. 4.

NUCB2/Nesfatin-1 knockdown (shNUCB2) MIN6 cells exhibit impaired GSIS and elevated UCP-2 mRNA expression. a MIN6 cells were introduced with shRNA silencing scramble (shScr) and with shRNAs silencing NUCB2 at two different nucleotide sequences (shNUCB2 seq 1 and shNUCB2 seq 2). NUCB2 protein expression was reduced in shNUCB2 seq 1 MIN6 cells compared to shScr MIN6 cells and mouse islets. b Stimulation with 16.6 mM glucose increased insulin secretion in shScr MIN6 cells but not in shNUCB2 seq 1 MIN6 cells under static incubation. c UCP-2 mRNA expression was elevated in shNUCB2 seq 1 MIN6 cells compared to shScr MIN6 cells