Fig. 2.

Involvement of PKC in the acute suppressive effect of IL-1β on channel activity. a Representative current trace showing that a PKC inhibitor, GF109203X, blocked the suppressive effect of IL-1β. b GF109203X restored the channel activity which was suppressed by IL-1β. The NP _o values calculated from these current traces were 0.78–0.83 for controls, 0.85 for GF109203X alone, 0.83 for GF109203X + IL-1β, 0 for IL-1β alone, and 0.64 for IL-1β + GF109203X. The doses of IL-1β and GF109203X were 15 pg/ml and 500 nM, respectively. Each trace was obtained from separate cell-attached patches at a V _p of 0 mV. c Summary of the effects of GF109203X on the IL-1β-induced suppression of channel activity. From the top, effects of GF109203X alone (n = 12), IL-1β in the presence of GF109203X (n = 12), IL-1β (n = 8), and GF109203X added after IL-1β (n = 8). **P < 0.01, significantly different compared with the initial control level