TABLE 1.
Bacterial strains, plasmids, and phages
| Strain, plasmid, or phage | Genotype | Source |
|---|---|---|
| S. peucetius | ||
| ATCC 29050 | Wild type | ATCCa |
| WMH1650 | dnrX aphII, obtained after infection with phWHM281 | This work |
| WMH1655 | dpsY aphII, obtained after infection with phWHM283 | This work |
| S. lividans | ||
| TK24 | SLP1− SLP2−, streptomycin resistant | 19 |
| WMH1594 | SLP1− SLP2−, streptomycin resistant, φC31 lysogen | 23 |
| Plasmids | ||
| pFDNeoS | pUC18 containing the aphII gene | 7 |
| pWHM249 | XhoI site eliminated from the aphII gene of pFDNeoS | 23 |
| pWHM335 | Cosmid clone containing a fragment of the DNR cluster | 40 |
| pWHM279 | 3.3-kb BamHI fragment cloned from pWHM335 in pUC18 | This work |
| pWHM280 | 1.0-kb SalI fragment with the aphII gene cloned blunt ended from pWHM249 into the filled-in NcoI site of pWHM279 | This work |
| pWHM282 | 1.0-kb SalI fragment with the aphII gene cloned blunt ended from pWHM249 into the filled-in EcoNI site of pWHM279 | This work |
| pWHM346 | 1.6-kb SstI-SphI fragment cloned from pWHM279 into pSP72 | This work |
| pWHM347 | 1.6-kb EcoRI-HindIII fragment cloned from pWHM346 into pWHM3 | This work |
| pWHM226 | 3.3-kb BamHI fragment cloned from pWHM279 into pWHM3 | This work |
| Phages | ||
| KC515 | c+ attP, thiostrepton resistant, viomycin resistant | 5 |
| phWHM281 | c+ attP, viomycin resistant; 4.3-kb BamHI fragment cloned from pWHM280 into KC515 between the BamHI and BglII sites | This work |
| phWHM283 | c+ attP, viomycin resistant; 4.3-kb BamHI fragment cloned from pWHM282 into KC515 between the BamHI and BglII sites | This work |
a
ATCC, American Type Culture Collection.