TABLE 2.
Anthracycline titers of S. peucetius 29050 (wild type), WMH1650, and WMH1655a
| Strain and compound | Titer (μg/ml)
|
|||||
|---|---|---|---|---|---|---|
| RHO | DNR | 13-dihydro-DNR | DXR | Xb | Yb | |
| 29050, no H3O+ | 0c | 6.1 | 0 | 8.8 | 6.8 | 5.6 |
| 29050 + H3O+ | 3.6 | 45.3 | 0 | 13.9 | 0 | 0 |
| WMH1650 (dnrX::aphII), no H3O+ | 0 | 35.4 | 18.0 | 41.3 | 0 | 0 |
| WMH1650 + H3O+ | 0 | 36.2 | 16.6 | 41.6 | 0 | 0 |
| WMH1655 (dpsY::aphII) | 0 | 0 | 0 | 0 | 0 | 0 |
a
Cultures were grown in APM medium for 120 h and worked up as described in Materials and Methods. The titers shown are typical of the values obtained in different fermentations.
b
X and Y, two different, unknown compounds with retention times of 35.9 and 39.3 min in the HPLC chromatogram.
c
No metabolite was found.