Fig. 4.

Effects of H₂O₂ on TRPV1. a Representative current trace (holding voltage −20 mV) showing response to 100 μM H₂O₂. Vertical lines reflect current responses to the repetitive ramp-like pulses from −80 to 80 mV. Right summary of I–V curved of control and H₂O₂-treated conditions (n = 5). Outwardly rectifying conductance was increased by H₂O₂. b An exemplary current trace at −20 mV holding voltage showing the repetitive activation of TRPV1 by 50 nM capsaicin. Then, 100 μM H₂O₂ was applied between the second and the fourth applications of capsaicin. Right summary of the capsaicin-induced inward currents normalized to the first response. I _TRPV1,Cap was not changed by H₂O₂ pretreatment (third bar, n = 10)