TABLE 2.

Properties of FecR′-BlaM hybrid proteins

Plasmid	Fusion site at amino acid	Size of hybrid protein (kDa)	Ampicillin resistance (μg/ml)	Growth of WA176	Cleavage of hybrid protein
pDW20		35.5	0	+	+
pDW26.08a	8	29.9	0	−	−
pDW26.21	21	31.5	0	−	−
pDW26.25	25	31.8	0	−	−
pDW26.61	61	35.8	0	+	−
pDW26.75	75	37.4	0	+	−
pDW26.78	78	37.7	0	+	−
pDW26.81	81	38.0	0	+	−
pDW26.107	107	41.0	200	+	−
pDW26.112	112	41.5	50	+	−
pDW26.127	127	43.2	100	+	−
pDW26.129	129	43.5	50	+	−
pDW26.133	133	43.9	100	+	−
pDW26.138	138	44.5	50	+	−
pDW26.149	149	45.7	50	+	−
pDW26.159	159	46.8	0	+	−
pDW26.172	172	47.9	5	+	−
pDW26.191	191	50.4	5	+	−
pDW26.209	209	52.4	0	+	−
pDW26.210	210	52.5	0	+	−
pDW26.230	230	54.8	100	+	+
pDW26.237	237	55.8	100	+	+
pDW26.245	245	56.4	200	+	+
pDW26.248	248	56.8	100	+	+
pDW26.257	257	57.8	100	+	+
pDW26.259	259	58.0	50	+	+
pDW26.265	265	58.7	10	+	+
pDW26.274	274	59.7	5	+	+
pDW26.279	279	60.2	5	+	+
pDW26.281	281	60.4	5	+	+
pDW26.301	301	62.7	5	+	+

^aIn this and the following plasmids, the last number denotes the number of N-terminal amino acids of FecR fused to mature BlaM.