TABLE 3.
FecR-BlaM activity in protease mutants and chaperone-overproducing cells
| Hybrid protein | Activitya in strain:
|
|||||
|---|---|---|---|---|---|---|
| KS272 (degP+) | KS474 (degP) | UT5600 (ompT) | 5K | 5K(pMS2) (groEL groES) | 5K(pDW102) (secB) | |
| FecR78-BlaM | 0 | 0 | 0 | 0 | 0 | 0 |
| FecR107-BlaM | 200 | 800 | 800 | 200 | 200 | 400 |
| FecR159-BlaM | 5 | 10 | 10 | 0 | 10 | 10 |
| FecR191-BlaM | 10 | 50 | 25 | 5 | 10 | 10 |
| FecR210-BlaM | 5 | 50 | 10 | 5 | NDb | ND |
| FecR265-BlaM | 10 | 25 | 50 | 10 | 25 | 25 |
a
The values denote the highest ampicillin concentrations (micrograms per milliliter) in nutrient agar plates on which the cells formed single colonies at 28°C.
b
ND, not determined.