Table 1.
Forward/reverse primers and PCR conditions for ACE, ACTN3, and PPARD genotyping
| Gene | Forward primer | Reverse primer | PCR reaction conditions | ||
|---|---|---|---|---|---|
| (5′–3′) | (5′–3′) | Denaturation | Annealing and cycles | Final extension | |
| ACE | CTGGAGACCACTCCCATCCTTTCT | GATGTGGCCATCACATTCGTCAGAT | 95°C for 5 min |
35 cycles of 95°C for 1 min, 58°C for 30 s, 72°C for 40 s |
72°C for 10 min |
| ACTN3 (exon 16) | CTGTTGCCTGTGGTAAGTGGG | TGGTCACAGTATGCAGGAGGG | 95°C for 5 min |
35 cycles of 95°C for 1 min, 58°C for 30 s, 72°C for 40 s |
72°C for 10 min |
| PPARD (exon 4) | CATGGTATAGCACTGCAGGAA | CTTCCTCCTGTGGCTGCTC | 95°C for 5 min |
35 cycles of 95°C for 1 min, 60°C for 30 s, 72°C for 40 s |
72°C for 10 min |