Fig. 7.

Localization of TRPC3 in mouse hearts. a Heart sections of Trpc3 ^−/− mice were stained with anti-TRPC3 antibodies (green) and anti-NK-ATPase antibodies (red) to confirm the staining specificity of anti-TRPC3 antibodies. b, c Double immunofluorescence images showing TRPC3 (green) and NK-ATPase (red) (b) or SERCA2 (red) (c) expression in ventricular cardiomyocytes of 129/Sv wild-type (WT) mice. The merged and magnified images show the small amount of co-localization of TRPC3 and NK-ATPase (b), compared to SERCA2 (c). The white inset is magnified in the right panel. Trpc3 ^−/− transient receptor potential canonical subtype 3 knockout, NK-ATPase sodium potassium ATPase, SERCA sarcoplasmic reticulum calcium ATPase