Fig. 1.

Hsa_circ_0136666 has intersection with miR-375, which positively associated with gastric cancer growth. a Hsa_circ_0136666 is enriched in differentially expressed genes in gene chip. b Schematic diagram of hsa_circ_0136666 biosynthesis. c Divergent primers were used for amplification, and PCR products were Sanger sequenced. d Hsa_circ_0136666 and linear-PRKDC gene expression abundance were detected under RNase digestion, n = 3. e The natural degradation rate of hsa_circ_0136666 and linear-PRKDC gene was detected under Actinomycin D treatment, n = 3. f Hsa_circ_0136666 and linear PRKDC genes were amplified using oligo dT and random primers, n = 3. g The expression of hsa_circ_0136666 in gastric cancer tissue sections was presented by FITC fluorescence. h Differential expression of hsa_circ_0136666 in tissues. Data are presented as the mean ± SD, Student's t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001