Fig. 5.

Hsa_circ_0136666 regulates immune responses through the miR-375/PRKDC signaling axis. a The gene chip overexpressing miR-375 enriched 262 downregulated genes and 1777 upregulated genes. b MiR-375 has complementary base pairing sites with wild-type PRKDC mRNA 3’UTR. c RIP experiment was used to verify the combination of miR-375 and PRKDC, n = 3. d Dual luciferase reporter assay was used to verify the interaction between PRKDC 3’UTR and miR-375, n = 6. e RNA in situ hybridization was implemented to verify the colocalization of PRKDC mRNA 3’UTR with miR-375. f Western blot was used to detect immune checkpoint proteins and PI3 signaling pathway proteins in MKN-45 cells while miR-375 overexpressing. g Original photo of tumor in tumor bearing mice under NU-7441 treatment. h Tumor growth curve of tumor bearing mice under NU-7441 treatment, n = 6. i Differential distribution of tumor infiltrating T lymphocytes in different groups of tumor bearing mice under NU-7441 treatment, n = 6. j Quantitative distribution of tumor infiltrating T lymphocytes in different groups, n = 6. Data are presented as the mean ± SD, Student's t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001